Journal: Gastroenterology

Article Title: Cysteine-rich domains of muc3 intestinal mucin promote cell migration, inhibit apoptosis, and accelerate wound healing.

doi: 10.1053/j.gastro.2006.09.006

Figure Lengend Snippet: Figure 6. Effect of Muc3 and MUC3A expression on apoptosis. (A) Western blotting using anti-Flag antibody. Lanes: stably transfected LoVo clone LhM3c14 cytoplasmic fraction (LhM3c14 cyt) and membrane fraction (LHM3C14 MEM), mock-transfected LoVo cell clone membrane fraction (LMOCK MEM), nontransfected LoVo membrane fraction (MEM). (B) Percentage change in apoptosis with () or without () TNF- (100 ng/mL) treatment for 48 hours. Cell lines included parental LoVo, LhM3c14, Lmock, and nontransfected LoVo cells pretreated with m3EGF1,2 (10 g/mL) or GST (5 g/mL) for 1 hour before addition of TNF-. Baseline apoptosis 3.9%. *P .016 vs LoVo (), ^P .009 vs GST, P .002 vs Lmock; n 2–3 plates/treatment and 3 fields counted/plate. (C) Percentage change in apoptosis in nontransfected LoVo cells with () or without () sequential interferon- and anti-Fas antibody treatment for 72 hours. Cells were pretreated with m3EGF1,2 (10 g/mL), m3EGF1 (10 g/mL), m3EGF2 (10 g/mL), GST (10 g/mL), EGF (10 ng/mL), or m3EGF1 (5 g/mL) m3EGF2 (5 g/mL) before additionofanti-Fasantibody.Baselineapoptosis1.1%and.9%forleftandrightpanels,respectively(ANOVA,P.001,withinbothpanels).*P.005vsLoVo, LoVo m3EGF1, m3EGF2 and GST controls (left panel). *P .004 vs LoVo and LoVo m3EGF1 m3EGF2 (right panel); n 2 plates/treatment and 2 fields counted/plate. (D) Percentage change in apoptosis with () or without () sequential interferon- and anti-Fas antibody treatment for 48 hours. Cell lines included LhM3c14andLmock(ANOVA,P.001).Baselineapoptosis2.3%.*P.025vsLmock()andLhMc14()n3plates/treatmentand3fieldscounted/plate. (E) LoVo cells were treated with increasing concentrations of the EGF-receptor inhibitor, tyrphostin (AG1478). This resulted in a dose-dependent reversal of the anti-apoptosis effect of recombinant EGF, but did not affect the anti-apoptosis effect of m3EGF1,2 (ANOVA, P .001). Baseline apoptosis 2.0%. *P .03 vs m3EGF1,2 (same tyrphostin concentrations). (Note that maximal apoptosis observed varied from experiment to experiment because of the limited half-life of the activity of the anti-Fas in storage).

Article Snippet: Apoptosis was measured in histologic specimens using a erminal deoxynucleotidyl transferase–mediated deoxyuridine riphosphate biotin nick-end labeling (TUNEL) assay (TACS.XL n Situ Apoptosis Detection Kit; R&D Systems), according to he manufacturer’s directions.

Techniques: Expressing, Western Blot, Stable Transfection, Transfection, Membrane, Recombinant, Activity Assay

Journal: Gastroenterology

Article Title: Cysteine-rich domains of muc3 intestinal mucin promote cell migration, inhibit apoptosis, and accelerate wound healing.

doi: 10.1053/j.gastro.2006.09.006

Figure Lengend Snippet: Figure 9. Effect of recombinant Muc3 protein on acetic acid–in- duced apoptosis. (A) Mean number of apoptotic cells determined by TUNEL assay in the distal colon of mice 30 hours after acetic acid administration in mice treated twice with 100 g m3EGF1,2 or control peptide 100 g BSA in PBS (n 10 mice each treatment). (B) Repre- sentative distal colon 30 hours after acetic acid and control enema treatments. TUNEL-positive nuclei are stained darkly. (C) Representa- tive distal colon 30 hours after acetic acid and m3EGF1,2 enema treat- ments (magnification, 200).

Article Snippet: Apoptosis was measured in histologic specimens using a erminal deoxynucleotidyl transferase–mediated deoxyuridine riphosphate biotin nick-end labeling (TUNEL) assay (TACS.XL n Situ Apoptosis Detection Kit; R&D Systems), according to he manufacturer’s directions.

Techniques: Recombinant, TUNEL Assay, Control, Staining

Journal: Scientific Reports

Article Title: MicroRNA410 Inhibits Pulmonary Vascular Remodeling via Regulation of Nicotinamide Phosphoribosyltransferase

doi: 10.1038/s41598-019-46352-z

Figure Lengend Snippet: miR410 overexpression increases hPAECs apoptosis. ( A ) Representative images of TUNEL positive cells (red), nucleus was stained by DAPI, and percentage of TUNEL positive cells was quantified in panel B. *P < 0.05, ***P < 0.001 relative to control; ### P < 0.001 relative to 50 nM group. Size bar: 20 µm.

Article Snippet: Three groups of cells cultured in 6-well plates were treated as described in the mitochondrial depolarization assay, then fixed with 4% paraform phosphate buffer saline, rinsed with PBS, then used in situ BrdU-Red DNA fragmentation (TUNEL) cell apoptosis detection kit (Abcam®, Cambridge, MA).

Techniques: Over Expression, TUNEL Assay, Staining